DOI: 10.12732/ijam.v38i3.8
APPLICATIONS OF CONTOURS AND
PIXEL VALUE SUMMATION IN DETERMINING
THREE-DIMENSIONAL SIGNAL INTENSITIES
Anoop Kishore1,*, Paka Sravan Kumar2,*, Kodi Triveni 3, Adarsh Gopinathan4, Nandakumar K5, Divya Shenoy Purushothama6,§
1,2,3,4,5 Department of Pharmacology
Manipal College of Pharmaceutical Sciences
Manipal Academy of Higher Education
Manipal - 576104, INDIA
6 Department of Mathematics
Manipal Institute of Technology
Manipal Academy of Higher Education
Manipal - 576104, INDIA
Abstract. Contours are two-dimensional plots of three-dimensional data. Contour lines and contour maps are useful mathematical tools for topographic mapping, geology, architecture, weather forecasting and several biological applications. In this paper their application has been extended to determining protein densities from biological samples. The proteins resolved using Western blot technique were subjected to contour-based band boundary determination combined with pixel-value based band density estimation. Additionally, the accuracy of this method has been evaluated by comparing it with the traditional densitometric analysis methods that use rolling disk algorithm that is used for estimating band density and background noise subtraction. It is demonstrated that the contour and pixel value technique is a versatile method for determining signals intensities from biological specimen.
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DOI: 10.12732/ijam.v38i3.8
Source: International Journal of Applied Mathematics
ISSN printed version: 1311-1728
ISSN on-line version: 1314-8060
Year: 2025
Volume: 38
Issue: 3
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